Production of a cloned calf from a fetal fibroblast cell line.
نویسندگان
چکیده
The present study examined the in vitro and in vivo development of bovine nuclear-transferred embryos. A bovine fetal fibroblast culture was established and used as nucleus donor. Slaughterhouse oocytes were matured in vitro for 18 h before enucleation. Enucleated oocytes were fused with fetal fibroblasts with an electric stimulus and treated with cytochalasin D and cycloheximide for 1 h followed by cycloheximide alone for 4 h. Reconstructed embryos were cultured for 7-9 days and those which developed to blastocysts were transferred to recipient cows. Of 191 enucleated oocytes, 83 (43.5%) were successfully fused and 24 (28.9%) developed to blastocysts. Eighteen freshly cloned blastocysts were transferred to 14 recipients, 5 (27.8%) of which were pregnant on day 35 and 3 (16.7%) on day 90. Of the three cows that reached the third trimester, one recipient died of hydrallantois 2 months before term, one aborted fetus was recovered at 8 months of gestation, and one delivered by cesarian section a healthy cloned calf. Today, the cloned calf is 15 months old and presents normal body development (378 kg) and sexual behavior (libido and semen characteristics).
منابع مشابه
Growth and Isolation of Human Cytomegalovirus on a New Human Fetal Foreskin Fibroblast-derived Cell Line in Iran
Cell culture technique has been used for detection and confirmation of many different viruses in clinical samples. Although, new diagnostic methods have been developed for viral infections, traditional cell culturetechnique is still regarded as the “gold standard” for several infectious agents such as human cytomegalovirus(HCMV). In the present study, a new human fetal foreskin fibr...
متن کاملO-12: Study of Expression of DevelopmentalGenes in SCNT Cloned Embryos
(SCNT) embryos of buffaloes. 2. To study gene expression profile of important developmental genes at different stages of SCNT cloned embryo. 3. To study epigenetic reprogramming during early developments of SCNT embryos Materials and Methods: Expression analysis of developmental genes was done in different (ovarian granulose and cumulus and skin fibroblasts) donor cells; in vitro maturing oocyt...
متن کاملSerum-dependent growth patterns of two, newly established human mesothelioma cell lines.
Two cell lines with different in vitro growth patterns were established from the pleural fluid of a patient with malignant epithelial pleural mesothelioma. The cell line established in RPMI 1640 supplemented with human AB serum had an epithelial morphology, while the cell line established in fetal calf serum-supplemented medium had a fibroblast-like morphology. Exposure of the fibroblast-like c...
متن کاملP-132: The Effect of Different Fetal Bovine Serum Levels on Recombinant Chinese Hamster Ovary Cells, Expressing HumanFollicle Stimulating Hormone
Background: Follicle stimulating hormone (FSH) is one of the most common and effective gonadotropin hormones used in infertility treatment. Production of FSH as a drug is affordable as long as it is done with minimal cost. FBS is the most expensive component in medium culture furthermore; it’s being controversial to cause unnecessary suffering for the unborn calf for collection their serum. In ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
دوره 36 11 شماره
صفحات -
تاریخ انتشار 2003